Reagents and instruments for immunology, cell biology and molecular biology.
 
> Dual Luciferase Reporter Gene Assay Kit

Dual Luciferase Reporter Gene Assay Kit

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Cat# KTA8010-100

Size : 100T

Brand : Abbkine

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Phone : +1 850 650 7790

Specification

Product name Dual Luciferase Reporter Gene Assay Kit
Applications notes Usually, the Transcriptional regulation element or 5'-promoter region of the gene of interest is cloned upstream of Luciferase, or the 3'- UTR region is cloned downstream of Luciferase to construct a Reporter gene plasmid, and then transfect the cell, treat the cell with appropriate drugs, and then Lytic cells. The Transcriptional regulation effect of drug treatment on the target gene is judged by detecting the activity of Luciferase.

Product Properties

Kit components •Lysis Buffer (5×)•Firefly Luciferase Assay Buffer•Firefly Luciferase Substrate•Renilla Luciferase Assay Buffer•Renilla Luciferase Substrate (50×)
Features & Benefits • High luminous intensity. • High detection sensitivity, wide linear range of the measured sample. • Easy operation, stable reading, fast speed. • Strong compatibility with various media.
Storage instructions Stored at -20℃ for 12 months, protected from light
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background In the presence of ATP, magnesium ions and oxygen, Firefly luciferase can catalyze firefly luciferin to Oxyluciferin, which generate light signals during the oxidation of luciferin. In the presence of oxygen, Renilla luciferase can catalyze the oxidation of coelenterazine to coelenteramide, and light signals are also generated during the oxidation of coelenterine. Through the Bioluminescence system of Luciferase and its substrate, gene expression can be detected very sensitively and efficiently.

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