E2F1 Transcription Factor (E2F1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3) (HRP)
Cat# MBS6243662-5x0,1mL
Size : 5x0.1mL
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
WB: Hela cell nuclear extract probed with E0010 at 1ug/ml.
Transcription Factor (E2F1) Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6243662MBS6243662 is an antibody targeting E2F1. Also known as E2F1 Transcription Factor (E2F1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3) (HRP). Reactive in Human, Xenopus. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
E2F1 Transcription Factor, Monoclonal Antibody
Full Product Name
E2F1 Transcription Factor (E2F1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3) (HRP)
Product Gene Name
anti-E2F1 antibody
Clonality
Monoclonal
Isotype
IgG
Clone Number
KH20 and KH95
Host
Mouse
Species Reactivity
Human, Xenopus
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes human E2F-1 at ~55kD. Species Crossreactivity: Xenopus
Preparation and Storage
Store at -20 degree C.
Molecular Weight
46,920 Da
OMIM
189971
Immunogen
GST fusion protein corresponding to full-length human E2F-1 (Accession #NP_005216). Epitope: aa1-89 for clone KH20; aa342-386 for clone KH95.
Conjugate
HRP
Product Categories/Family for anti-E2F1 antibody
Antibodies; Transcription Factors
Applications Tested/Suitable for anti-E2F1 antibody
Immunoprecipitation (IP), Western Blot (WB)
Product References and Citations for anti-E2F1 antibody
1. Helin, K., et al., (1993) Genes Dev. 7:1850-1861. 2. Wu, C.L., et al., (1995) Mol. Cell. Biol. 15:2536-2546. 3. Iwase, S., et al. (1997) J Biol Chem 272: 12406-14. 4. Wu, L. et al (2000). Mol Cell Biol 20: 7059-67.
Other Notes
Small volumes of anti-E2F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for E2F1. It may not necessarily be applicable to this product.
NCBI Official Full Name
transcription factor E2F1
NCBI Official Synonym Full Names
E2F transcription factor 1
NCBI Official Symbol
E2F1
NCBI Official Synonym Symbols
RBP3; E2F-1; RBAP1; RBBP3
UniProt Protein Name
Transcription factor E2F1
UniProt Synonym Protein Names
PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
Protein Family
Transcription factor
UniProt Gene Name
E2F1
UniProt Synonym Gene Names
RBBP3; E2F-1; RBAP-1; RBBP-3
UniProt Entry Name
E2F1_HUMAN
NCBI Protein Information
transcription factor E2F1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; retinoblastoma-associated protein 1; retinoblastoma-binding protein 3
UniProt Comments for E2F1
E2F1: a member of the E2F/DP family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Binds DNA cooperatively with Dp transcription factors in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.The Dp-1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It can mediate both cell proliferation and p53-dependent apoptosis.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity
Biological Process: Notch signaling pathway; transcription, DNA-dependent; apoptosis; positive regulation of transcription, DNA-dependent; mRNA stabilization; negative regulation of transcription from RNA polymerase II promoter; anoikis; positive regulation of fibroblast proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of transcription, DNA-dependent; DNA damage checkpoint; forebrain development; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle
Show more Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity
Biological Process: Notch signaling pathway; transcription, DNA-dependent; apoptosis; positive regulation of transcription, DNA-dependent; mRNA stabilization; negative regulation of transcription from RNA polymerase II promoter; anoikis; positive regulation of fibroblast proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of transcription, DNA-dependent; DNA damage checkpoint; forebrain development; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-E2F1 antibody
| Reference Product | PubMed Publications |
|---|---|
| MYC antibody | >222 publications with E2F1 and MYC |
| E2F4 antibody | >190 publications with E2F1 and E2F4 |
| E2F2 antibody | >159 publications with E2F1 and E2F2 |
| CDKN2A antibody | >136 publications with E2F1 and CDKN2A |
| TFDP1 antibody | >89 publications with E2F1 and TFDP1 |
| SP1 antibody | >82 publications with E2F1 and SP1 |
| ATM antibody | >52 publications with E2F1 and ATM |
| RBL1 antibody | >38 publications with E2F1 and RBL1 |
| RBL2 antibody | >37 publications with E2F1 and RBL2 |
| CDC25A antibody | >36 publications with E2F1 and CDC25A |
Pathways associated with anti-E2F1 antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Activation Of BH3-only Proteins Pathway antibodies | Activation Of BH3-only Proteins Pathway Diagram |
| Activation Of NOXA And Translocation To Mitochondria Pathway antibodies | Activation Of NOXA And Translocation To Mitochondria Pathway Diagram |
| Activation Of PUMA And Translocation To Mitochondria Pathway antibodies | Activation Of PUMA And Translocation To Mitochondria Pathway Diagram |
| Adipogenesis Pathway antibodies | Adipogenesis Pathway Diagram |
| Apoptosis Pathway antibodies | Apoptosis Pathway Diagram |
| Assembly Of The Pre-replicative Complex Pathway antibodies | Assembly Of The Pre-replicative Complex Pathway Diagram |
| Association Of Licensing Factors With The Pre-replicative Complex Pathway antibodies | Association Of Licensing Factors With The Pre-replicative Complex Pathway Diagram |
| Bladder Cancer Pathway antibodies | Bladder Cancer Pathway Diagram |
| Bladder Cancer Pathway antibodies | Bladder Cancer Pathway Diagram |
| CDC6 Association With The ORC:origin Complex Pathway antibodies | CDC6 Association With The ORC:origin Complex Pathway Diagram |
Diseases associated with anti-E2F1 antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >971 publications with E2F1 and Neoplasms |
| Breast Neoplasms Antibodies | >137 publications with E2F1 and Breast Neoplasms |
| Cell Transformation, Neoplastic Antibodies | >129 publications with E2F1 and Cell Transformation, Neoplastic |
| Adenocarcinoma Antibodies | >128 publications with E2F1 and Adenocarcinoma |
| Neoplasms, Experimental Antibodies | >109 publications with E2F1 and Neoplasms, Experimental |
| Nervous System Diseases Antibodies | >89 publications with E2F1 and Nervous System Diseases |
| Lung Neoplasms Antibodies | >82 publications with E2F1 and Lung Neoplasms |
| Liver Neoplasms Antibodies | >75 publications with E2F1 and Liver Neoplasms |
| Prostatic Neoplasms Antibodies | >72 publications with E2F1 and Prostatic Neoplasms |
| Liver Diseases Antibodies | >70 publications with E2F1 and Liver Diseases |
Organs/Tissues associated with anti-E2F1 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Lung Antibodies | >145 publications with E2F1 and Lung |
| Blood Antibodies | >132 publications with E2F1 and Blood |
| Brain Antibodies | >130 publications with E2F1 and Brain |
| Liver Antibodies | >105 publications with E2F1 and Liver |
| Muscle Antibodies | >96 publications with E2F1 and Muscle |
| Prostate Antibodies | >89 publications with E2F1 and Prostate |
| Skin Antibodies | >68 publications with E2F1 and Skin |
| Bone Antibodies | >55 publications with E2F1 and Bone |
| Embryonic Tissue Antibodies | >51 publications with E2F1 and Embryonic Tissue |
| Kidney Antibodies | >43 publications with E2F1 and Kidney |