EGFR Protein, Human, Recombinant (His)

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Cat# NB-64-55948-5ug

Size : 5ug

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EGFR Protein, Human, Recombinant (His)

(Synonyms: PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor) Copy Product Info

Synonyms: PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor

Catalog No. TMPY-00742 Copy Product Info

EGFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 69.8 kDa and the accession number is P00533-1.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 98.5%; SEC-HPLC: 93.3%

Appearance:Lyophilized powder

Color:White

Product Introduction

Bioactivity

Bioactivity	1.Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc at 2 μg/ml (100 μl/well) can bind Human EGFR His , the EC50 of Human EGFR His is 100-500 ng/mL. 2. Captured Cetuximab (IgG1) on proA Chip can bind Human EGFR recombinant protein with an affinity constant of 1.07 nM as determined in an SPR assay (Routinely tested). 3. Loaded Cetuximab on ProA Biosensor, can bind Human EGFR protein, His Tag with an affinity constant of 5.81nM as determined in BLI assay (Routinely tested).
Description	EGFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 69.8 kDa and the accession number is P00533-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P00533-1
Construction	A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Leu 25
Protein Purity	> 95 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor
Research Background	The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

Chemical Properties

Molecular Weight	69.8 kDa (predicted); 110 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

PIG-61PIG 61NISBD-2NISBD 2HER-1HER 1ERBB-1ERBB 1

Related Tags: EGFR Protein, Human, Recombinant (His) chemical structure | EGFR Protein, Human, Recombinant (His) molecular weight