F-Actin Polyclonal Antibody
Cat# bs-1571R
Size : 100uL
Brand : Bioss
Contact local distributor :
Phone : +1 850 650 7790
Formalin-fixed and paraffin embedded rat skeletal muscle labeled with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 and L2 rat skeletal muscle lysates probed: : Anti-F-Actin (bs-1571R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa
A549 cell lysates probed with Anti-F-Actin Polyclonal Antibody, Unconjugated (bs-1571R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1571r |
| Product Name | F-Actin Polyclonal Antibody |
| Applications | WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC) |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human beta-Actin |
| Immunogen Range | 1-50/374 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 60 |
| Swiss Prot | P60709 |
| Subcellular location | Cytoplasm |
| Synonyms | CapZ alpha-1; F-actin-capping protein subunit alpha-1; CAZA1_HUMAN |
| Background | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
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