FAS Antibody
Cat# OAEE01009
Size : 100tests(T100)
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for FAS Antibody |
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| Predicted Species Reactivity | Human |
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| Product Format | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Clonality | Monoclonal |
| Clone | LT95 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | APC |
| Application | FC |
| Reconstitution and Storage | 2°C to 8°C |
| Immunogen | HUT-78 human T cell lymphoma cell line |
| Purification | Purified by size-exclusion chromatography |
| Concentration | 10 ul / test |
| Specificity | The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule. |
| Formulation | APC |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
| Reference | Drosopoulos KG, Roberts ML, Cermak L, Sasazuki T, Shirasawa S, Andera L, Pintzas A.: Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway. J Biol Chem. 2005 Jun 17;280(24):22856-67. Epub 2005 Mar 8. Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res. 2008 Feb 1;314(3):554-63. Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic-cellsecretion of CC and CXC chemokines. Blood. 2005 Sep 15;106(6):2033-41. Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol. 2003 Jun 15;170(12):6209-16. Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 1998 Mar 16;17(6):1675-87. |
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| Gene Symbol | FAS |
| Gene Full Name | Fas cell surface death receptor |
| Alias Symbols | ALPS1A, APO-1, Apo-1 antigen, APO-1 cell surface antigen, apoptosis antigen 1, apoptosis signaling receptor FAS, apoptosis-mediating surface antigen FAS, APT1, CD95, CD95 antigen, Fas (TNF receptor superfamily, member 6), Fas AMA, FAS1, FASLG receptor, FASTM, mutant tumor necrosis receptor superfamily member 6, TNF receptor superfamily member 6, TNFRSF6, tumor necrosis factor receptor superfamily member 6, tumor necrosis factor receptor superfamily, member 6. |
| NCBI Gene Id | 355 |
| Protein Name | Tumor necrosis factor receptor superfamily member 6 |
| Description of Target | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). |
| Uniprot ID | P25445 |
| Protein Accession # | NP_000034.1; NP_690610.1; NP_690611.1 |
| Nucleotide Accession # | NM_000043.4; NM_152871.2; NM_152872.2 |
