StorageThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).
Detection Method:Sandwich Test principle:This assay employs a two-site sandwich ELISA to quantitate FSH in samples. An antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FSH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
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et al,NEURODEGENERATIONInOnby et al..PMID:", (2023), PMID: 37156043
et al,Reproductive endocrine disruption and gonadal intersex induction in male Japanese medaka chronically exposed to betamethasone at environmentally relevant levelsInJ Hazard MaterOn2023 Aug 5byMaoliang Su , Youling Zhong et al..PMID:37156043, (2023), PMID: 37156043
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