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- Product Name
- FITC Anti-Mouse CD5 Antibody(53-7.3)
- Catalogue No.
- FITC-30201
- Form
- liquid
- Conjugation
- FITC
- Conjugation Information
- FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).
- Clonality
- Monoclonal
- Isotype
- IgG2a, κ
- Clone ID
- 53-7.3
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- CD5|Cd5|Ly-1|Lymphocyte antigen 1|Lyt-1 antibody
- UniProt ID
- P13379
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
C57BL/6 murine splenocytes are stained with APC Anti-Mouse CD3 Antibody and FITC Anti-Mouse CD5 Antibody (Left). Splenocytes are stained with APC Anti-Mouse CD3 Antibody and FITC Rat IgG2a, κ Isotype Control (Right). - Background
- CD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α/β T cells express high levels of CD5, very few γ/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.