General Ethinylestradiol (EE) Mini Samples ELISA Kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ethinylestradiol in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to ethinylestradiol has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ethinylestradiol and unlabeled ethinylestradiol (Standards or samples) with the pre-coated antibody specific to ethinylestradiol. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ethinylestradiol in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ethinylestradiol in the sample.

Small volumes of EE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2088089 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ethinylestradiol (EE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EE. The ELISA analytical biochemical technique of the MBS2088089 kit is based on EE antibody-EE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).