Glycogen Synthase Kinase 3 beta (GSK3b)
Cat# G8170-50K-100ug
Size : 100ug
Brand : US Biological
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Phone : +1 850 650 7790
G8170-50KGlycogen Synthase Kinase 3 beta (GSK3b)
Specifications
Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3α and GSK-3β) share 85% amino acid identity. GSK-3β, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.
Hybridoma:
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived recombinant human Glycogen Synthase Kinase-3 beta (rhGSK-3β; aa 1-420; Accession # P49841).
Applications:
Immunocytochemistry
Recommended Dilution:
Immunocytochemistry: This antibody was used at a concentration of 25ug/mL with the appropriate secondary reagents to detect GSK-3β in fixed human MCF-7 and HeLa cells. For chromogenic detection of labeling, the use of Cell and Tissue Staining Kits is recommended.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.