gp100 (95kD melanocyte-specific Secreted GlycoProtein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte Protein Pmel 17, Melanoma Associated ME20 antigen, Melanosomal matrix Protein17, p100, p26, PMEL17, Premelanosome Protein, Secreted melanoma-Associated ME20 antigen, SILV, Silver Homolog, Melanosome) (FITC)

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control:
SK-MEL-28 cells or Melanoma

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.