Reagents and instruments for immunology, cell biology and molecular biology.
 
> H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein, Mouse, MHC (His & Avi), Biotinylated

H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein, Mouse, MHC (His & Avi), Biotinylated

Print Print
Please log in

Cat# TMPK-01956-200ug

Size : 200ug

Brand : TargetMol

Contact local distributor :


Phone : +1 850 650 7790

H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein, Mouse, MHC (His & Avi), Biotinylated

(Synonyms: OVA, MHC, H2-K1, H-2K) Copy Product Info
H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein, Mouse, MHC (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P01901(H-2K(b))&P01887(B2M)&SIINFEKL.
H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein, Mouse, MHC (His & Avi), Biotinylated
For research use only—not for human use. No sales to individuals. Use as intended only.

Resource Download

User Guide of Recombinant Proteins

Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it.
Description
H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein, Mouse, MHC (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P01901(H-2K(b))&P01887(B2M)&SIINFEKL.
Species
Mouse
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP01901(H-2K(b))&P01887(B2M)&SIINFEKL
ConstructionHis24-Pro297 (H-2K(b)), Ile21-Met119 (B2M) and SIINFEKL peptide
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin< 1 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsOVA, MHC, H2-K1, H-2K
Research Background
Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
Chemical Properties
Molecular Weight50.20 kDa (Predicted); 52-70 kDa (Reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.