Herpes Simplex Virus 1 (HSV 1) IgG ELISA Kit

Intended Uses: The HSV 1 IgG ELISA is intended for the determination of IgG class antibodies against Herpes simplex Virus 1 (HSV 1) in human serum or plasma (citrate, heparin). For research use only -

Principle of the Assay: The immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Im-munosorbent Assay) technique. Microtiterplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound con-jugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction prod-uct. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sul-phuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450/620 nm is read using an ELISA Microtiterplate reader.

Small volumes of Herpes Simplex Virus 1 (HSV 1) IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS495630 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Herpes Simplex Virus 1 (HSV 1) IgG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Herpes Simplex Virus 1 (HSV 1) IgG. The ELISA analytical biochemical technique of the MBS495630 kit is based on Herpes Simplex Virus 1 (HSV 1) IgG antibody-Herpes Simplex Virus 1 (HSV 1) IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Herpes Simplex Virus 1 (HSV 1) IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Herpes Simplex Virus 1 (HSV 1) IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).