Background: SARS-CoV-2 is an enveloped virus with a positive-sense RNA genome and a nucleocapsid of helical symmetry. The SARS-CoV-2 entry into host cells is mediated by the transmembrane spike (S) glycoprotein.

Principle of the Assay: 96-well plates are coated with SARS-CoV-2 S1 protein that captures IgG antibodies against SARS- CoV-2 S1 protein in the sample. After washing away unbound materials, captured IgG against SARS-CoV-2 S1 protein is detected by anti-human IgG polyclonal antibodies conjugated with horse radish peroxidase (HRP). After washing step, the chromogenic substrate 3,3',5,5'- tetramethylbenzidine (TMB) is added. Color reaction is stopped by 2M H2SO4. The amount of anti- SARS- CoV-2 S1 antibodies captured inside the wells is proportional to the color density generated in the coupled oxidation-reduction reaction. The unknown sample concentration can be interpolated from a standard curve generated by Human anti-S1 monoclonal antibody (mAb).

Intended Uses: SARS-CoV-2 S1 protein IgG ELISA Kit is a highly sensitive and specific immunoassay for the precision detection and quantitative measurement of IgG class antibodies against the spike S1 protein of SARS-CoV-2 virus in human blood samples in vitro.

Small volumes of COVID-19 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS398013 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the COVID 19 high sensitivity Spike S1 IgG Coronavirus (COVID-19) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COVID-19. The ELISA analytical biochemical technique of the MBS398013 kit is based on COVID-19 antibody-COVID-19 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COVID-19 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COVID-19. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).