Histone H3, phosphorylated (Ser28)
Cat# MBS630703-0,02mL
Size : 0.02mL
Brand : MyBiosource
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Phone : +1 850 650 7790
Western blot analysis of lysates from CHO and HeLa cells either untreated or synchronized in metaphase by treatment with 0.1 mg/ml nocodazole for 4 h, followed by isolation of metaphase cells by mitotic shake-off. Blots were probed with MBS630703 (upper) or H5110-13D (lower).
Flow cytometric analysis of untreated Jurkat cells, using MBS630703 versus propidium iodide (DNA content). The box indicates phospho-Histone H3 positive cells.
Confocal immunofluorescent analysis of C2C12 cells using MBS630703(green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye)
Confocal immunofluorescent analysis of postnatal day 1 rat brain using MBS630703(green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Histone H3 (H3) Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630703Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-terminal tails… More details
Product Overview
Online Datasheet in PDFProduct Name
Histone H3, Polyclonal Antibody
Full Product Name
Histone H3, phosphorylated (Ser28)
Product Gene Name
anti-H3 antibody
Product Synonym Names
Anti -Histone H3, phosphorylated (Ser28)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Drosophila, Hamster, Human, Mouse
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Specificity
Recognizes endogenous levels of histone H3 only when phosphorylated at Ser28. Does not cross-react with other phosphorylated histones, including phospho-histone H3 (Ser10). Species crossreactviity
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide (KLH-coupled) corresponding to the amino terminus of histone H3 phosphorylated on Ser28.
Product Categories/Family for anti-H3 antibody
Antibodies; Abs to Histones
Applications Tested/Suitable for anti-H3 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-H3 antibody
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Flow cytometry.
Dilution: Western blot: 1:1000
Immunoprecipitation: 1:25
Immunofluorescence (IF-F, IF-IC): 1:400
Flow Cytometry: 1:200
Dilution: Western blot: 1:1000
Immunoprecipitation: 1:25
Immunofluorescence (IF-F, IF-IC): 1:400
Flow Cytometry: 1:200
Western Blot (WB) of anti-H3 antibody
Western blot analysis of lysates from CHO and HeLa cells either untreated or synchronized in metaphase by treatment with 0.1 mg/ml nocodazole for 4 h, followed by isolation of metaphase cells by mitotic shake-off. Blots were probed with MBS630703 (upper) or H5110-13D (lower).
Other Notes
Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for H3 purchase
MBS630703 is an antibody targeting H3. Also known as Histone H3, phosphorylated (Ser28). Reactive in Drosophila, Hamster, Human, Mouse. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.
GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
Protein Family
Tyrosine phosphatase
NCBI Protein Information
histone H3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Organs/Tissues associated with anti-H3 antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Bone Antibodies | >13 publications with H3 and Bone |
| Connective Tissue Antibodies | >4 publications with H3 and Connective Tissue |
| Heart Antibodies | >3 publications with H3 and Heart |
| Muscle Antibodies | >3 publications with H3 and Muscle |
| Brain Antibodies | >3 publications with H3 and Brain |
| Vascular Antibodies | >2 publications with H3 and Vascular |
| Embryonic Tissue Antibodies | >2 publications with H3 and Embryonic Tissue |
| Ear Antibodies | >1 publications with H3 and Ear |
| Kidney Antibodies | >1 publications with H3 and Kidney |
| Umbilical Cord Antibodies | >1 publications with H3 and Umbilical Cord |