Histone H3, phosphorylated (Thr3)

Cat# MBS625079-5x0,1mL

Size : 5x0.1mL

Brand : MyBiosource

Contact local distributor :


Phone : +1 850 650 7790

Histone H3 (H3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS625079

MBS625079 is an antibody targeting H3. Also known as Histone H3, phosphorylated (Thr3). Reactive in Human. Host: Rabbit. More details

Product Overview

Online Datasheet in PDF
Product Name
Histone H3, Polyclonal Antibody
Full Product Name
Histone H3, phosphorylated (Thr3)
Product Gene Name
anti-H3 antibody
Product Synonym Names
Anti -Histone H3, phosphorylated (Thr3)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in 0.05% sodium azide and 30% glycerol.
Purity/Purification
Serum
Immunodepleted rabbit antiserum
Specificity
Recognizes Histone H3 phosphorylated at Thr3, Mr ~17kD. Does not crossreact with peptides
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
KLH-conjugated, synthetic peptide containing (AR[pT]KQ…), in which pT corresponds to phospho-threonine at position 3 of human Histone H3.

Product Categories/Family for anti-H3 antibody

Antibodies; Abs to Histones
Applications Tested/Suitable for anti-H3 antibody
Western Blot (WB)
Application Notes for anti-H3 antibody
Suitable for use in Immunoblot.
Dilution: Western Blot: 1:5,000-1:10,000 detects phosphorylated Histone H3 in acid extracted
proteins from mitotic HeLa cells but not unphosphorylated recombinant Histone H3. Recombinant Histone H3 protein and acid extract from colcemid-treated HeLa cells were resolved by electrophoresis, transferred to nitrocellulose and probed with a 1:5,000 dilution of antiphospho-Histone H3 (Thr3). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Western Blot (WB) of anti-H3 antibody

WB: Recombinant Histone H3 protein (lane 1) and acid extract from colcemid-treated HeLa cells (lane 2) were probed with a 1:5,000 dilution of MBS625079-Histone H3 (Thr3).
Rabbit Anti-H3 Polyclonal Antibody – Cat# MBS625079

Immunocytochemistry (ICC) of anti-H3 antibody

ICC: Mitotic mouse fibroblast cells were stained with MBS625079
Rabbit Anti-H3 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS625079

Other Notes

Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
Protein Family
Tyrosine phosphatase
NCBI Protein Information
histone H3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-H3 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with H3 and Bone
 Connective Tissue Antibodies  >4 publications with H3 and Connective Tissue
 Heart Antibodies  >3 publications with H3 and Heart
 Muscle Antibodies  >3 publications with H3 and Muscle
 Brain Antibodies  >3 publications with H3 and Brain
 Vascular Antibodies  >2 publications with H3 and Vascular
 Embryonic Tissue Antibodies  >2 publications with H3 and Embryonic Tissue
 Ear Antibodies  >1 publications with H3 and Ear
 Kidney Antibodies  >1 publications with H3 and Kidney
 Umbilical Cord Antibodies  >1 publications with H3 and Umbilical Cord