HLA-DQA1 Antibody - Biotin Conjugated
Cat# OACA02038
Size : 100ug
Brand : Aviva Systems Biology
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HLA-DQA1 Antibody - Biotin Conjugated (OACA02038)
| Datasheets/Manuals | Printable datasheet for HLA-DQA1 Antibody - Biotin Conjugated (OACA02038) (OACA02038) |
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| Predicted Species Reactivity | Human |
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| Product Format | Liquid |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Biotin |
| Application | ELISA, IHC |
| Reconstitution and Storage | -20°C or -80°C |
| Immunogen | Recombinant Human HLA class II histocompatibility antigen, DQ alpha 1 chain protein (24-213AA) |
| Purification | Protein G Purified |
| Purity | Caprylic Acid Ammonium Sulfate Precipitation purified |
| Storage Buffer | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reference | [1] The heavy chain of human B-cell alloantigen HLA-DS has a variable N-terminal region and a constant immunoglobulin-like region.'Chang H.-C., Moriuchi T., Silver J.Nature 305:813-815(1983) [2] Both alpha and beta chains of HLA-DC class II histocompatibility antigens display extensive polymorphism in their amino-terminal domains.'Schenning L., Larhammar D., Bill P., Wiman K., Jonsson A.-K., Rask L., Peterson P.A.EMBO J. 3:447-452(1984) [3] Isotypic and allotypic variation of human class II histocompatibility antigen alpha-chain genes.'Auffray C., Lillie J.W., Arnot D., Grossberger D., Kappes D., Strominger J.L.Nature 308:327-333(1984) |
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| Gene Symbol | HLA-DQA1 |
| Gene Full Name | major histocompatibility complex, class II, DQ alpha 1 |
| Alias Symbols | CELIAC1;DC-1 alpha chain;DC-alpha;DQA1;DQ-A1;HLA class II histocompatibility antigen DQ alpha chain;HLA class II histocompatibility antigen, DQ alpha 1 chain;HLA-DCA;HLA-DQA;MHC class II antigen DQA1;MHC class II DQ alpha chain;MHC class II DQA1;MHC class II HLA-DQ-alpha-1;MHC class II protein;MHC HLA-DQ alpha;truncated MHC class II antigen. |
| NCBI Gene Id | 3117 |
| Protein Name | HLA class II histocompatibility antigen, DQ alpha 1 chain |
| Description of Target | Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. |
| Uniprot ID | P01909 |
