Immunohistochemistry staining with anti-human HLA-G (MEM-G/1) - pulmonary disseases (paraffin-embedded sections). The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.Immunohistochemistry staining with anti-human HLA-G (MEM-G/1) of first trimester placenta (paraffin-embedded sections).
View other "HLA-G or NP_002118.1" antibodies
cDNA Clones
Lentiviral
AAV
CRISPR
RNAi
Proteins
Antibodies
Specifications
Product Data
Clone Name
MEM-G/1
Application
IHC, WB
Recommended Dilution
Western Blotting. Recommended dilution 1-2 μg/ml. Immunohistochemistry on Paraffin Sections: 1/60-1/100 Incubation Time: 1 hour at RT. Positive tissue: Human placenta - extravillous cytotrophoblast. Staining technique: Standard ABC technique. Pretreatment: Heat retrieval in 0.01 M citrate buffer (4x2 min. in microwave oven).
Reactivity
Human
Antibody Host
Mouse
Isotype
IgG1
Clonality
Monoclonal
Immunogen
Recombinant Human HLA-G denaturated heavy chain
Specificity
The antibody reacts with denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Buffer
Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
Synonyms
HLA-6.0, HLAG, MHC class I antigen G
Reference Data
Protein Categories
Allergies and autoimmune diseases, Cytokines, Membrane Proteins, Secreated Proteins
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