HN1 siRNA (Human)
Cat# MBS828410-15nmol
Size : 15nmol
Brand : MyBiosource
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Phone : +1 850 650 7790
HN1 siRNA – Hematological and Neurological Expressed 1 Protein Isoform 2 (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828410MBS828410 is an siRNA targeting HN1. Also known as HN1 siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit HN1 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
HN1, siRNA
Full Product Name
HN1 siRNA (Human)
Product Gene Name
HN1 sirna
Product Synonym Names
ARM2; Hematological and neurological expressed 1 protein; Androgen-regulated protein 2
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
HN1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
11,023 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for HN1 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of HN1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for HN1. It may not necessarily be applicable to this product.
NCBI Official Full Name
hematological and neurological expressed 1 protein isoform 2
NCBI Official Synonym Full Names
hematological and neurological expressed 1
NCBI Official Symbol
HN1
NCBI Official Synonym Symbols
ARM2; HN1A
UniProt Protein Name
Hematological and neurological expressed 1 protein
UniProt Synonym Protein Names
Androgen-regulated protein 2
Protein Family
Hematological and neurological expressed 1 protein
UniProt Gene Name
HN1
UniProt Synonym Gene Names
ARM2
UniProt Entry Name
HN1_HUMAN
NCBI Protein Information
hematological and neurological expressed 1 protein
UniProt Comments for HN1
HN1: a nuclear protein of the HN1 family. Upregulated in advanced-stage epithelial ovarian cancer. Three alternatively spliced isoforms have been described.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: nucleus
Biological Process: developmental process
Show more Protein type: Unknown function
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: nucleus
Biological Process: developmental process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with HN1 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Apoptosis Modulation And Signaling Pathway antibodies | Apoptosis Modulation And Signaling Pathway Diagram |
Diseases associated with HN1 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >6 publications with HN1 and Nervous System Diseases |
| Disease Models, Animal Antibodies | >3 publications with HN1 and Disease Models, Animal |
| Adenocarcinoma Antibodies | >2 publications with HN1 and Adenocarcinoma |
| Poisoning Antibodies | >2 publications with HN1 and Poisoning |
| Liver Diseases Antibodies | >2 publications with HN1 and Liver Diseases |
| Neoplasms, Experimental Antibodies | >2 publications with HN1 and Neoplasms, Experimental |
| Cell Transformation, Neoplastic Antibodies | >1 publications with HN1 and Cell Transformation, Neoplastic |
| Neurobehavioral Manifestations Antibodies | >1 publications with HN1 and Neurobehavioral Manifestations |
| Carcinoma, Hepatocellular Antibodies | >1 publications with HN1 and Carcinoma, Hepatocellular |
| Liver Neoplasms Antibodies | >1 publications with HN1 and Liver Neoplasms |
Organs/Tissues associated with HN1 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >9 publications with HN1 and Brain |
| Prostate Antibodies | >4 publications with HN1 and Prostate |
| Thymus Antibodies | >3 publications with HN1 and Thymus |
| Intestine Antibodies | >3 publications with HN1 and Intestine |
| Embryonic Tissue Antibodies | >3 publications with HN1 and Embryonic Tissue |
| Blood Antibodies | >3 publications with HN1 and Blood |
| Liver Antibodies | >3 publications with HN1 and Liver |
| Lung Antibodies | >3 publications with HN1 and Lung |
| Connective Tissue Antibodies | >2 publications with HN1 and Connective Tissue |
| Vascular Antibodies | >2 publications with HN1 and Vascular |