RayBio® C-Series Human Angiogenesis Antibody Array 1000 Kit. A combination of Human Angiogenesis Antibody Array C1 & C2. Detects 43 Human Angiogenic Factors. Suitable for all liquid sample types.
Best Seller
Human Angiogenesis Array C1000
Human Angiogenesis Array C1000
Contact Us for Bulk Request
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 43 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Angiogenin
| Angiopoietin-1
| Angiopoietin-2
| Angiostatin
| bFGF
|
EGF
| ENA-78 (CXCL5)
| Endostatin
| GCSF
| GM-CSF
|
GRO alpha/beta/gamma
| I-309 (TCA-3/CCL1)
| IFN-gamma
| IGF-1
| IL-10
|
IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-2
| IL-4
| IL-6
|
IL-8 (CXCL8)
| I-TAC (CXCL11)
| Leptin
| MCP-1 (CCL2)
| MCP-3 (MARC/CCL7)
|
MCP-4 (CCL13)
| MMP-1
| MMP-9
| PDGF-BB
| PECAM-1 (CD31)
|
PLGF
| RANTES (CCL5)
| TGF beta 1
| Thrombopoietin (TPO)
| Tie-2
|
TIMP-1
| TIMP-2
| TNF alpha
| uPAR
| VEGF-A
|
VEGFR2
| VEGFR3
| VEGF-D
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Angiogenesis Antibody Array C1000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
Excel (AAH-ANG-1_Analysis_Tool.xls)
Excel (AAH-ANG-2_Analysis_Tool.xls)
citations
Studying sprouting angiogenesis under combination of oxygen gradients and co-culture of fibroblasts using microfluidic cell culture model
- Species:
- Human
- Sample Type:
- human umbilical vein endothelial cells (HUVECs) , MRC-5 cell
- Year:
- 2023
- Journal Name:
- Materials Today Bio
The Carotenoid Diatoxanthin Modulates Inflammatory and Angiogenesis Pathways In Vitro in Prostate Cancer Cells
- Species:
- Human
- Sample Type:
- PC3 cell
- Year:
- 2023
- Journal Name:
- Antioxidants
Anti-Vascular Endothelial Growth Factor Therapy Abolishes Glioma-Associated Endothelial Cell-Induced Tumor Invasion
- Species:
- Human
- Sample Type:
- Human Umbilical Vein Endothelial Cell (HUVEC), U87 cell
- Year:
- 2023
- Journal Name:
- Journal of Molecular Neuroscience
Anticancer Effects of Fucoxanthin through Cell Cycle Arrest, Apoptosis Induction, and Angiogenesis Inhibition in Triple-Negative Breast Cancer Cells
- Species:
- Human
- Sample Type:
- MDA-MB-231 Cell
- Year:
- 2023
- Journal Name:
- Molecules
FGF10/FGFR2 Signaling: Therapeutically Targetable Vulnerability in Ligand-responsive Cholangiocarcinoma Cells
- Species:
- Human
- Sample Type:
- KKU-M213A cell
- Year:
- 2023
- Journal Name:
- In Vivo
Exploring association of melanoma-specific Bcl-xL with tumor immune microenvironment
- Species:
- Human
- Sample Type:
- M14 cell
- Year:
- 2023
- Journal Name:
- Journal of Experimental & Clinical Cancer Research
Higher Expression of Annexin A2 in Metastatic Bladder Urothelial Carcinoma Promotes Migration and Invasion
- Species:
- Human
- Sample Type:
- T24 cell
- Year:
- 2022
- Journal Name:
- Cancers
Sanguinarine Inhibition of TNF-α-Induced CCL2, IKBKE/NF-κB/ERK1/2 Signaling Pathway, and Cell Migration in Human Triple-Negative Breast Cancer Cells
- Species:
- Human
- Sample Type:
- MDA-MB-231 cell, MDA-MB-468 cell
- Year:
- 2022
- Journal Name:
- International Journal of Molecular Sciences
Co-targeting triple-negative breast cancer cells and endothelial cells by metronomic chemotherapy inhibits cell regrowth and migration via downregulation of the FAK/VEGFR2/VEGF axis and autophagy/apoptosis activation
- Species:
- Human
- Sample Type:
- MDA-MB-231 cell, BT-549 cell
- Year:
- 2022
- Journal Name:
- Frontiers in oncology
Epoxyeicosatrienoic acid (EET)-stimulated angiogenesis is mediated by epoxy hydroxyeicosatrienoic acids (EHETs) formed from COX-2
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2019
- Journal Name:
- Journal of Lipid Research
- PubMed ID:
- 31641036