Principle of the Assay: This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus E8L antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox virus IgG (E8L) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox virus IgG (E8L). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox virus IgG (E8L) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Intended Uses: Used for the quantitative determination of Human Anti-Monkeypox virus IgG (E8L) concentration in serum and plasma.

Expression, purification and characterization of C2 domain of milk fat globule-EGF-factor 8-L. PMID: 17049264
Rational development of multicomponent mRNA vaccine candidates against mpox. PMID: 36947428
MFG-E8 in the retina and retinal pigment epithelium of rat and mouse. PMID: 17167398
Inhibition of monkeypox virus replication by RNA interference. PMID: 19889227
In silico designing a novel TLR4-mediating multiepitope vaccine against monkeypox via advanced immunoinformatics and bioinformatics approaches. PMID: 37129119

Small volumes of E8L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1580024 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Monkeypox Virus (MPXV) IgG (E8L) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing E8L. The ELISA analytical biochemical technique of the MBS1580024 kit is based on E8L antibody-E8L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect E8L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, E8L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).