Membrane-based antibody array for detection 43 apoptosis biomarkers, including Caspase 3, Caspase 8, bad, bax, bcl-2, etc.
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Human Apoptosis Array C1
Human Apoptosis Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 43 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Apoptosis, IGF Signaling, PI3K-AKT Signaling, p53 Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| bad
| bax
| bcl-2
| bcl-w
| BID
|
| BIM
| Caspase-3
| Caspase-8
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
|
| cIAP-2
| Cytochrome C
| DR6 (TNFRSF21)
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
|
| HSP27
| HSP60
| HSP70
| HTRA2
| IGF-1
|
| IGF-2
| IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
|
| IGFBP-5
| IGFBP-6
| IGF-1 R
| livin
| p21
|
| p27
| p53
| SMAC
| Survivin (BIRC5)
| TNF RI (TNFRSF1A)
|
| TNF RII (TNFRSF1B)
| TNF alpha
| TNF beta (TNFSF1B)
| TRAIL R1 (TNFRSF10A/DR4)
| TRAIL R2 (TNFRSF10B/DR5)
|
| TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| XIAP
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Apoptosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-κB Translocation Inhibitor in SKOV-3 Cell Line
- Species:
- Human
- Sample Type:
- SKOV-3 cell
- Year:
- 2023
- Journal Name:
- International Journal of Nanomedicine
Specific Forms of Graphene Quantum Dots Induce Apoptosis and Cell Cycle Arrest in Breast Cancer Cells
- Species:
- Human
- Sample Type:
- MCF-7 cell, BT-474 cell，T-47D cell，MDA-MB-231 cell
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
Polyphenolic Carbon Quantum Dots with Intrinsic Reactive Oxygen Species Amplification for Two-Photon Bioimaging and In Vivo Tumor Therapy
- Species:
- Mouse
- Sample Type:
- B16F10 cell
- Year:
- 2023
- Journal Name:
- Acs Applied Materials & Interfaces
Novel combination treatment of CDK 4/6 inhibitors with PARP inhibitors in triple negative breast cancer cells
- Species:
- Human
- Sample Type:
- HCC1937 cell, HCC1937-R cell
- Year:
- 2023
- Journal Name:
- Naunyn-schmiedebergs Archives of Pharmacology
Koenimbin, a natural dietary compound of Murraya koenigii (L) Spreng: inhibition of MCF7 breast cancer cells and targeting of derived MCF7 breast cancer stem cells (CD44+/CD24−/low): an in vitro study
- Species:
- Human
- Sample Type:
- MCF7 cell
- Year:
- 2023
- Journal Name:
- Drug Design Development and Therapy
In vitro and in vivo anti-angiogenic activity of girinimbine isolated from Murraya koenigii
- Species:
- Human
- Sample Type:
- human umbilical vein endothelial cells (HUVECs)
- Year:
- 2023
- Journal Name:
- Drug Design Development and Therapy
Citicoline Eye Drops Protect Trabecular Meshwork Cells from Oxidative Stress Injury in a 3D In Vitro Glaucoma Model
- Species:
- Human
- Sample Type:
- trabecular meshwork (TM) cell
- Year:
- 2022
- Journal Name:
- International Journal of Molecular Sciences
Statins inhibit proliferation and induce apoptosis in triple-negative breast cancer cells
- Species:
- Human
- Sample Type:
- SUM159 cell
- Year:
- 2022
- Journal Name:
- Medical Oncology
Knockdown of CHPF suppresses cell progression of non-small-cell lung cancer
- Species:
- Human
- Sample Type:
- A549 cell
- Year:
- 2022
- Journal Name:
- Cancer management and research
α-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-κB and Hsp70 protein modulation in vitro, and tumor reduction in vivo
- Species:
- Human
- Sample Type:
- MCF-7 cell
- Year:
- 2022
- Journal Name:
- Drug Design Development and Therapy