Human Autophagy Related Protein 5 (ATG5) ELISA Kit
Cat# MBS2706900-5x96
Size : 5x96-Strip-Wells
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Autophagy Related Protein 5 (ATG5) ELISA Kit for Human
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2706900SPECIAL PROMOTIONAL PRICING for a limited time
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATG5 in human serum, plasma, tissue homogenates and other biological fluids.Principle of the Assay: The microplate provided in this kit has been pre-coated with… More details
Product Overview
Online Datasheet in PDFProduct Name
Autophagy Related Protein 5 (ATG5), ELISA Kit
Full Product Name
Human Autophagy Related Protein 5 (ATG5) ELISA Kit
Product Gene Name
ATG5 elisa kit
Product Synonym Names
ASP; APG5; APG5L; hAPG5; Apoptosis-specific protein; Autophagy protein 5
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.78-50ng/mL
Sensitivity
< 0.28ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of ATG5. No significant cross-reactivity or interference between ATG5 and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATG5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATG5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
39,687 Da
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Other Notes
Small volumes of ATG5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ATG5 purchase
MBS2706900 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Autophagy Related Protein 5 (ATG5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATG5. The ELISA analytical biochemical technique of the MBS2706900 kit is based on ATG5 antibody-ATG5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATG5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATG5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for ATG5. It may not necessarily be applicable to this product.
NCBI Official Full Name
Autophagy-related protein 5
NCBI Official Symbol
NCU04662
UniProt Protein Name
Autophagy-related protein 5
Protein Family
Autophagy protein
UniProt Gene Name
apg-4
UniProt Synonym Gene Names
atg5
NCBI Protein Information
autophagy protein 5
UniProt Comments for ATG5
Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy). Also required for mitophagy, which eliminates defective or superfluous mitochondria in order to fulfill cellular energy requirements and prevent excess ROS production. Conjugation with atg12, through a ubiquitin-like conjugating system involving apg-5/atg7 as an E1-like activating enzyme and atg10 as an E2-like conjugating enzyme, is essential for its function. The atg12-apg-4/atg5 conjugate acts as an E3-like enzyme which is required for lipidation of apg-6/atg8 and apg-6/atg8 association to the vesicle membranes ().
Show more Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with ATG5 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Regulation Of Autophagy Pathway antibodies | Regulation Of Autophagy Pathway Diagram |
| Regulation Of Autophagy Pathway antibodies | Regulation Of Autophagy Pathway Diagram |
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