Quantibody® Human Bone Metabolism Array 1000 Kit. Detects 41 Human Cytokines. Suitable for all liquid sample types.
Human Bone Metabolism Array Q1000
Human Bone Metabolism Array Q1000
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 41 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Energy Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 100 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Activin A
| aFGF (FGF-1)
| Amphiregulin
| bFGF
| BMP-4
|
BMP-9
| E-Selectin
| ICAM-1 (CD54)
| IGF-1
| IL-1 alpha (IL-1 F1)
|
IL-1 beta (IL-1 F2)
| IL-6
| IL-8 (CXCL8)
| IL-11
| IL-17A
|
MCP-1 (CCL2)
| M-CSF
| MIP-1 alpha (CCL3)
| MMP-2
| MMP-9
|
MMP-13
| Osteoactivin (GPNMB)
| P-Cadherin
| RANK (TNFRSF11A)
| SDF-1 alpha (CXCL12 alpha)
|
Sonic Hedgehog N-Terminal (Shh-N)
| TGF beta 1
| TGF beta 2
| TNF alpha
| VCAM-1 (CD106)
|
VE-Cadherin (CDH5)
| BMP-2
| BMP-6
| BMP-7
| DKK-1
|
MMP-3
| Osteoprotegerin (TNFRSF11B)
| Osteopontin (SPP1)
| PDGF-BB
| TGF beta 3
|
TRANCE (TNFSF11)
| ||||
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Bone Metabolism Array Q1000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-BMA-1.gal)
GAL (GAL-QAH-BMA-2.gal)
Excel (QAH-BMA-1_Analysis_Tool.xls)
Excel (QAH-BMA-2_Analysis_Tool.xls)