AAH-CYT-3-4 | Human Cytokine Array C3 Biovalley

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Human Cytokine Array C3

RayBio^® C-Series Human Cytokine Antibody Array 3 Kit. Detects 42 Humans Cytokines. Suitable for all liquid sample types.

Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	42
Recommended Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Shipping Type	Blue ice
Storage	-20°C

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	GRO alpha/beta/gamma Gene Symbol: CXCL1/CXCL2/CXCL3 Gene ID: 2919/2920/2921 Accession #: P09341/P19875/P19876	GRO alpha (CXCL1) Gene Symbol: CXCL1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA
I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-3 Gene Symbol: IL3 Gene ID: 3562 Accession #: P08700 ELISA Kit: RayBio Human IL-3 ELISA
IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA
IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-12 p40/p70 Gene Symbol: IL12A; IL12B Accession #: P29459	IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA
MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MCP-2 (CCL8) Gene Symbol: CCL8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA	MCP-3 (MARC/CCL7) Gene Symbol: CCL7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603 ELISA Kit: RayBio Human M-CSF ELISA	MDC (CCL22) Gene Symbol: CCL22 Gene ID: 6367 Accession #: O00626 ELISA Kit: RayBio Human MDC ELISA
MIG (CXCL9) Gene Symbol: CXCL9 Gene ID: 4283 Accession #: Q07325 ELISA Kit: RayBio Human MIG ELISA	MIP-1 delta (CCL15) Gene Symbol: CCL15 Gene ID: 6359 Accession #: Q16663 ELISA Kit: RayBio Human MIP-1 delta ELISA	RANTES (CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	SCF Gene Symbol: KITLG Gene ID: 4254 Accession #: P21583 ELISA Kit: RayBio Human SCF ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 alpha ELISA
TARC (CCL17) Gene Symbol: CCL17 Gene ID: 6361 Accession #: Q92583 ELISA Kit: RayBio Human TARC ELISA	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA
IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA	Angiogenin Gene Symbol: ANG Gene ID: 283 Accession #: P03950 ELISA Kit: RayBio Human Angiogenin ELISA	Oncostatin M Gene Symbol: OSM Gene ID: 5008 Accession #: P13725 ELISA Kit: RayBio Human OSM ELISA	Thrombopoietin (TPO) Gene Symbol: THPO Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA	VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA
PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	Leptin Gene Symbol: LEP Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA

Typical Data

RayBiotech’s Human Cytokine Antibody C3 Array (Cat# AAH-CYT-3-8) was used to show that progesterone selectively suppressed pCTS-L–induced secretion of TNF, IL-10, ENA-78, and MCP-1 in primary human PBMCs. Figure adapted from Qiang et al., Frontiers in Immunology, 2024.

RayBiotech’s Human Cytokine Antibody Array C3 (Cat# AAH-CYT-3) was used to analyze secreted cytokine profiles in L-02 liver cells exposed to chronic ethanol release. The array revealed dynamic changes in angiogenin, M-CSF, IL-3, G-CSF, IL-8, and VEGF-A levels. Figure adapted from Kim et al., Journal of Biological Engineering, 2024.

RayBiotech’s Human C3 Cytokine Array (Cat# AAH-CYT-3) was used to show that S. pneumoniae stimulation induced IL-6, M-CSF, and GROα/β/γ release from human brain pericytes. Figure adapted from Teske et al., Journal of Neuroinflammation, 2023.

RayBiotech’s Human Chemokine Antibody Array C1 (Cat# AAH-CHE-G1) was used to show that ETV2 overexpression upregulated CXCL5 secretion in HUVECs. Figure adapted from Sun et al., International Journal of Molecular Sciences, 2023.

RayBiotech’s Human Cytokine Antibody Array C3 (Cat# AAH-CYT-3) was used to show that lanosterol-loaded nanoparticles reduced the release of inflammatory proteins such as TNF, IL-6, and chemokines from human immune cells stimulated with procathepsin L. Figure adapted from Chen et al., International Journal of Molecular Sciences, 2023.

Kit Components

Human Cytokine Antibody Array C3 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Additional Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

documents

Datasheet (AAH-CYT-3.pdf)

SDS (AAH-CYT-3.pdf)

Excel (AAH-CYT-3_Analysis_Tool.xls)

citations

ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16INK4a levels
Species:
Human
Sample Type:
IMR90 cell
Year:
2022
Journal Name:
Nature Cell Biology
Ectosomal PKM2 Promotes HCC by Inducing Macrophage Differentiation and Remodeling the Tumor Microenvironment
Year:
2020
Journal Name:
Molecular Cell
PubMed ID:
32470318
Extracellular Vesicles from Pancreatic Cancer Stem Cells Lead an Intratumor Communication Network (EVNet) to fuel tumour progression
Species:
Human
Sample Type:
PaCa-2 cell
Year:
2022
Journal Name:
Gut
Therapeutic potential of procathepsin L-inhibiting and progesterone-entrapping dimethyl-β-cyclodextrin nanoparticles in treating experimental sepsis
Year:
2024
Journal Name:
Frontiers in immunology
PubMed ID:
38550581
Mimicking chronic alcohol effects through a controlled and sustained ethanol release device
Year:
2024
Journal Name:
Journal of biological engineering
PubMed ID:
38715085
Pericytes are protective in experimental pneumococcal meningitis through regulating leukocyte infiltration and blood-brain barrier function
Year:
2023
Journal Name:
Journal of Neuroinflammation
PubMed ID:
37978545
Cross Talk between the TM4SF5/Focal Adhesion Kinase and the Interleukin-6/STAT3 Pathways Promotes Immune Escape of Human Liver Cancer Cells
Species:
Human
Sample Type:
hepatocytes, HCC827 cell, SNU449 cell, SNU761 cell
Year:
2023
Journal Name:
Molecular and Cellular Biology
ETV2 Enhances CXCL5 Secretion from Endothelial Cells, Leading to the Promotion of Vascular Smooth Muscle Cell Migration
Species:
Human
Sample Type:
Human umbilical vein endothelial cells (HUVECs)
Year:
2023
Journal Name:
International Journal of Molecular Sciences
Development of Procathepsin L (pCTS-L)-Inhibiting Lanosterol-Carrying Liposome Nanoparticles to Treat Lethal Sepsis
Year:
2023
Journal Name:
International Journal of Molecular Sciences
PubMed ID:
37239992
Influence of Wound Fluid on the Transdifferentiation of Human Mesenchymal Bone Marrow Stem Cells into Cancer-Associated Fibroblasts
Species:
Human
Sample Type:
mesenchymal stem cells (MSC)
Year:
2023
Journal Name:
Cells Tissues Organs

Human Cytokine Array C3

Cat# AAH-CYT-3-4

Contact local distributor :

Brand : RayBiotech

Contact local distributor :

Human Cytokine Array C3

Product Description

Specifications

Product Features

Target Names

Typical Data

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

documents

citations

ADAR1 downregulation by autophagy drives senescence independently of RNA editing by enhancing p16INK4a levels

Ectosomal PKM2 Promotes HCC by Inducing Macrophage Differentiation and Remodeling the Tumor Microenvironment

Extracellular Vesicles from Pancreatic Cancer Stem Cells Lead an Intratumor Communication Network (EVNet) to fuel tumour progression

Therapeutic potential of procathepsin L-inhibiting and progesterone-entrapping dimethyl-β-cyclodextrin nanoparticles in treating experimental sepsis

Mimicking chronic alcohol effects through a controlled and sustained ethanol release device

Pericytes are protective in experimental pneumococcal meningitis through regulating leukocyte infiltration and blood-brain barrier function

Cross Talk between the TM4SF5/Focal Adhesion Kinase and the Interleukin-6/STAT3 Pathways Promotes Immune Escape of Human Liver Cancer Cells

ETV2 Enhances CXCL5 Secretion from Endothelial Cells, Leading to the Promotion of Vascular Smooth Muscle Cell Migration

Development of Procathepsin L (pCTS-L)-Inhibiting Lanosterol-Carrying Liposome Nanoparticles to Treat Lethal Sepsis

Influence of Wound Fluid on the Transdifferentiation of Human Mesenchymal Bone Marrow Stem Cells into Cancer-Associated Fibroblasts

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