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Human Endocan ELISA Kit
Human Endocan ELISA Kit
RayBio® Human Endocan ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 3.5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 3.5 ng/ml - 1000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human B7-2 (CD86), BAFF R (TNFRSF13C), Calcitonin, Calsyntenin-1, Cathepsin E, cIAP-2 (HIAP-1), Coagulation Factor VII, Complement MASP3, EphA2, EphB4, Ephrin-A4, FGF-23, FGF-5, Flt-3 (Flk-2), GLP-1 (7-37 & 7-36- NH2), Glypican 2, GM-CSF R alpha, GP73 (GOLM1), HTRA2 (Omi), IL-20 R alpha, IL-4 R alpha, JAM-C, Luteinizing Hormone (LH), Matrilin-3, Meprin alpha (MEP1A), MSP R (Ron), N-Cadherin, Neprilysin-2 (MMEL1), NKp44, Pappalysin-1 (PAPP-A), Parathyroid hormone (PTH), Pepsinogen II (PGC), Peptide YY (PYY), Presenilin 1 (PSEN1), SOX2, TFPI-2, TRACP (ACP5), Trefoil factor 3 (TFF3), Ubiquitin+1. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 5 fold |
| Accession Number | Q9NQ30 |
| Gene Id | 11082 |
| Gene Symbols | ESM1 |
| Protein Name / Synonyms | Endothelial cell-specific molecule 1 (ESM-1) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Related Products
NexaTag® Human Endocan qIPCR ELISA Kit
As low as $779.00
RayPlex® Human Endocan Bead Assay
As low as $240.00
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human Endocan ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Endocan (ESM1), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
Correlation analysis of the expression levels of serum endothelial cell adhesion molecule-1 and sirtuin 1 protein in acute respiratory distress syndrome
- Species:
- Human
- Sample Type:
- Serum (ARDS patients)
- Year:
- 2026
- Journal Name:
- Journal of medical biochemistry
- PubMed ID:
- 42781202
Endocan Participates in TGF-β/Smad and AKT/ERK Signaling Coordination and lncRNA Modulation in Human Cardiac Fibroblasts
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (TGF-β-treated cardiac fibroblasts)
- Year:
- 2026
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 42737642
Endocan level and its association with glycemic and inflammatory indices in diabetic nephropathy
- Species:
- Human
- Sample Type:
- Serum (T2DM with/without DN, controls)
- Year:
- 2026
- Journal Name:
- BMC endocrine disorders
- PubMed ID:
- 41998583
Endocan Knockdown Down-Regulates the Expression of Angiogenesis-Associated Genes in Il-1ß Activated Chondrocytes
- Species:
- Human
- Sample Type:
- chondrocytes
- Year:
- 2023
- Journal Name:
- Biomolecules
Endocan Promotes Pro-Tumorigenic Signaling in Lung Cancer Cells: Modulation of Cell Proliferation, Migration and lncRNAs H19 and HULC Expression
- Species:
- Human
- Sample Type:
- A549 Cell
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
