Human Epstein-barr virus IgG (EBv-IgG) ELISA Kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with EBv-Ag. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Anti-Human IgG and incubated. Then substrate solutions are added to each well. Only those wells that contain EBv-Ab-IgG and enzyme-conjugated Anti-Human IgG will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

Intended Uses: For the qualitative detection of Human Epstein-barr virus IgG antibody (EBv-Ab-IgG) concentration in serum, plasma and other biological fluids.

Small volumes of EBv-IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285442 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Epstein-barr virus IgG (EBv-IgG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EBv-IgG. The ELISA analytical biochemical technique of the MBS285442 kit is based on EBv-IgG antibody-EBv-IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EBv-IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EBv-IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).