General Information
| Short Description | This FGF23 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | Capture immunogen: Recombinant Human FGF23 protien.Detection Immunogen: Recombinant Human FGF23 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases. O-glycosylated at Thr-171 and Thr-178 by GALNT3 and glycosylation of Thr-178 requires previous glycosylation at Thr171. Glycosylation is necessary for secretion.it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. Phosphorylation at Ser-180 mediated by FAM20C slows down glycosylation at Thr-178 notably. |
| Function | Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Up-regulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. |
| Protein Name | Fibroblast Growth Factor 23Fgf-23PhosphatoninTumor-Derived Hypophosphatemia-Inducing Factor Cleaved Into - Fibroblast Growth Factor 23 N-Terminal Peptide - Fibroblast Growth Factor 23 C-Terminal Peptide |
| Database Links | |
| Cellular Localisation | SecretedSecretion Is Dependent On O-Glycosylation |
| Alternative Antibody Pair Names | Fibroblast Growth Factor 23 antibody pairFgf-23 antibody pairPhosphatonin antibody pairTumor-Derived Hypophosphatemia-Inducing Factor Cleaved Into - Fibroblast Growth Factor 23 N-Terminal Peptide - Fibroblast Growth Factor 23 C-Terminal Peptide antibody pairFGF23 antibody pairHYPF antibody pairUNQ3027 antibody pairPRO9828 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human FGF23 (Fibroblast Growth Factor 23) Antibody Pair (STJAP00422)
This antibody pair is designed to detect human FGF23 (Fibroblast Growth Factor 23), corresponding to UniProt entry Q9GZV9. Reactivity has been confirmed for human samples only.
Both antibodies are goat-derived and antigen affinity purified, supplied at 0.5 mg/mL. The capture antibody is unconjugated and formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is biotinylated and formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This pair has been validated for use in ELISA. It is suitable for detection of FGF23 in serum, plasma, and other biological fluids.
Both the capture and detection antibodies were raised against recombinant human FGF23 protein. No additional immunogen region data is provided in the product specifications.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.