Orb1807464-96Tests | Human Heat Shock Protein 90 (HSP-90) ELISA Kit

Human Heat Shock Protein 90 (HSP-90) ELISA Kit

SKU: orb1807464

Description

Human Heat Shock Protein 90 (HSP-90) ELISA Kit

Research Area

Metabolism Research

Images & Validation

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Application Notes	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-90. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-90, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSP-90. You can calculate the concentration of Human HSP-90 in the samples by comparing the OD of the samples to the standard curve.

Application Notes

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-90. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-90 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-90, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HSP-90. You can calculate the concentration of Human HSP-90 in the samples by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, Tissue homogenate and Other biological samples
Assay Type	Sandwich
Assay Time	3.5H
Range	3.13-200ng/mL
Sensitivity	1.21 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Hsp9

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Human Heat Shock Protein 90 (HSP90) ELISA Kit [orb779144]
Reactivity:
Human
Dynamic Range:
3.13-200 ng/mL
Sensitivity:
1.22 ng/mL
Sizes Available: 48 T, 96 T
Human Heat Shock Protein HSP 90-Alpha (HSP90α) ELISA Kit [orb2667357]
Reactivity:
Human
Dynamic Range:
1.56-100 ng/mL
Sensitivity:
0.53 ng/mL
Sizes Available: 48 T, 96 T
Human HSP90AA1 ELISA Kit [orb562445]
Reactivity:
Human
Dynamic Range:
3.125-200ng/ml
Sensitivity:
1.875ng/ml
Sizes Available: 96 T, 48 T
FS-Human HSP-90(Heat Shock Protein 90) ELISA Kit [orb3192911]
Reactivity:
Human
Dynamic Range:
3.13-200ng/mL
Sensitivity:
0.96ng/mL
Sizes Available: 48 T, 96 T
MF-Human HSP90AA1(Heat shock protein HSP 90-alpha) ELISA Kit [orb3206677]
Reactivity:
Human
Dynamic Range:
1.56-100ng/mL
Sensitivity:
0.71ng/mL
Sizes Available: 48 T, 96 T

Quick Database Links

Gene Symbol

HSP90

UniProt

P08238

UniProt Details

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No UniProt data available

Human Heat Shock Protein 90 (HSP-90) ELISA Kit

Cat# orb1807464-96Tests

Contact local distributor :

Brand : Biorbyt

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Human Heat Shock Protein 90 (HSP-90) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

Human Heat Shock Protein 90 (HSP90) ELISA Kit [orb779144]

Human Heat Shock Protein HSP 90-Alpha (HSP90α) ELISA Kit [orb2667357]

Human HSP90AA1 ELISA Kit [orb562445]

FS-Human HSP-90(Heat Shock Protein 90) ELISA Kit [orb3192911]

MF-Human HSP90AA1(Heat shock protein HSP 90-alpha) ELISA Kit [orb3206677]

Quick Database Links

UniProt Details

Protocol Information

Reactivity:	Human
Dynamic Range:	3.13-200 ng/mL
Sensitivity:	1.22 ng/mL