RayBio® Human IL-18 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Human IL-18 ELISA Kit
Human IL-18 ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 0.5 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.5 pg/ml - 75 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 5 - 50 fold |
| Accession Number | Q14116 |
| Gene Id | 3606 |
| Gene Symbols | IL18, IGIF, IL1F4 |
| Protein Name / Synonyms | Interleukin-18 (IL-18) (Iboctadekin) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Target Background
Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines. Similar to IL-1b, it is synthesized as an inactive precursor which requires processing by caspase-1. However, unlike IL-1b, IL-18 is constitutively active in nearly all healthy cells. IL-18 binding protein (IL-18BP) has high affinity for IL-18, thus mediating the activity of IL-18. IL-18 forms a signaling complex with the IL-18Rα receptor and IL-18Rβ co-receptor to mediate NF?B pro-inflammatory signaling. IL-18BP, present in the extracellular compartment, binds mature IL-18 preventing binding to the IL-18 receptor. IL-18 has been implicated in conditions such as autoimmune disease, myocardial infarction, metabolic syndromes, inflammatory bowel disease and psoriasis.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
citations
Interleukin-18 and Gelsolin Are Associated with Acute Kidney Disease after Cardiac Catheterization
- Species:
- Human
- Sample Type:
- urinary
- Year:
- 2023
- Journal Name:
- Biomolecules
Skin allergenicity of airborne and soil algae isolated from Malaysia
- Species:
- Human
- Sample Type:
- NCTC 2544 cell
- Year:
- 2023
- Journal Name:
- Aerobiologia
Low molecular weight cytotoxic components (DAMPs) form the post-COVID-19 syndrome
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Immunobiology
Comparative Evaluation of Gingival Crevicular Fluid Interleukin-17, 18 and 21 in Different Stages of Periodontal Health and Disease
- Species:
- Human
- Sample Type:
- gingival crevicular fluid (GCF)
- Year:
- 2022
- Journal Name:
- Medicina-lithuania
Altered inflammasome machinery as a key player in the perpetuation of Rett syndrome oxinflammation
- Species:
- Human
- Sample Type:
- Serum (female patients with clinical diagnosis of typical RTT and MECP2 mutation and healthy controls)
- Year:
- 2020
- Journal Name:
- Redox biology
- PubMed ID:
- 31606551
Shiori Yoshikawa, Sachiyo Yoshio, Yuichi Yoshida, Yuriko Tsutsui, Hironari Kawai, Taiji Yamazoe, Taizo Mori, Yosuke Osawa, Masaya Sugiyama, Masashi Iwamoto, Koichi Watashi, Takumi Kawaguchi, Tomoyuki Akita, Junko Tanaka, Yoshimi Kikuchi, Masashi Mizokami, Shinichi Oka, Tatsuya Kanto, Hiroyuki Gatanaga, Impact of Immune Reconstitution-Induced Hepatic Flare on Hepatitis B Surface Antigen Loss in Hepatitis B Virus/Human Immunodeficiency Virus-1 Coinfected Patients, The Journal of Infectious Diseases, , jiaa662, https://doi.org/10.1093/infdis/jiaa662
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2020
- Journal Name:
- The Journal of Infectious Diseases
NLRP3 inflammasome blockade reduces adipose tissue inflammation and extracellular matrix remodeling
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2019
- Journal Name:
- Cellular & Molecular Immunology
Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis
- Species:
- Human/mouse
- Sample Type:
- (HK-2 Cells)
- Year:
- 2018
- Journal Name:
- Redox biology
NLRP3 inflammasome inhibition attenuates silica-induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells
- Species:
- Human
- Sample Type:
- Conditioned Media (Human Bronchial epithelial cells in silica model)
- Year:
- 2018
- Journal Name:
- Experimental Cell Research
- PubMed ID:
- 29258746
Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Macrophages that don't express or have SHP2 blocked)
- Year:
- 2017
- Journal Name:
- Nature Communications
- PubMed ID:
- 29255148