Human Induced Pluripotent Stem Cells (mRNA) f_FAA

Cat# iPS210

Size : 5x105cells/vial

Brand : Alstem

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Human Induced Pluripotent Stem Cells (mRNA) f_FAA

Catalog Number iPS210
Unit 5x105 cells/vial
Shipping Condition Dry Ice - Overnight Shipping
Volume
5x105 cells/vial

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Cat no. iPS210

Highlights

Footprint-free human iPS210 cell lines generated using mRNA are ideally suited for various research purpose including 1) differentiating various somatic cells or organoid models for phenotypic and target-based compound screening, 2) establishing genetically modified disease model through CRISPR/Cas9 editing, and 3) generating functional cells/tissues as regenerative biology initiatives. iPS210 has several features such as:

  • Low passage and long-term viability
  • Off the shelf - simple thaw the cells and plate them onto serum-free, feeder-free culture
  • Transgene- and Virus-free
  • Homogeneity– Originated from a single iPSC clone

description

Footprint-free human iPS (induced pluripotent stem) cell line was derived from human fibroblasts (female, African American) by ectopic expression of OCT4, SOX2, KLF4, and c-MYC genes using mRNA. The cells were derived using morphological selection criteria and without the use of fluorescent markers or drug selection. When cultured under standard human ES cell culture conditions, the morphology of human iPS cells is identical to that of human ES cells. The cells also express the pluripotency markers SSEA-4 and NANOG and demonstrate strong endogenous alkaline phosphatase activity.

specification

Product Name Human Induced Pluripotent Stem Cells (mRNA) f_FAA
Shipping Condition

Dry Ice - Overnight Shipping

Storage and Stability

Store in vapor phase of liquid nitrogen immediately upon receipt. This product is stable for 12 months when stored as directed.

Quality Control

Human iPS cells (cat. no. iPS210) were grown in mTeSR1 medium. Each lot of human iPS cells is tested for growth and viability following recovery from cryopreservation. In addition, each lot is tested for expression of OCT4 and TRA-1-60, as well as the activity of alkaline phosphatase.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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