Glass slide multiplex ELISA array quantitatively detects 10 human inflammatory factors.
Human Inflammation Array Q1
Human Inflammation Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Inflammation, JAK/STAT Signaling, MAPK Signaling, NFkB Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
| IL-13
|
IL-4
| IL-6
| IL-8 (CXCL8)
| MCP-1 (CCL2)
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Inflammation Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-INF-1.gal)
Excel (QAH-INF-1_Analysis_Tool.xls)
citations
Androgen Receptor Activation Induces Senescence in Thyroid Cancer Cells
- Species:
- Human
- Sample Type:
- 8505C cell
- Year:
- 2023
- Journal Name:
- Cancers
Bacterial distribution and inflammatory cytokines associated with oral cancer with and without jawbone invasion—a pilot study
- Species:
- Human
- Sample Type:
- mucosa tissue, oral squamous cell carcinomas (OSCCs) tissue
- Year:
- 2023
- Journal Name:
- Clinical Oral Investigations
Lipopolysaccharide induces skin scarring through the TLR4/Myd88 inflammatory signaling pathway in dermal fibroblasts
- Species:
- Human
- Sample Type:
- normal skin derived fibroblasts (NSFs)
- Year:
- 2023
- Journal Name:
- Burns
miR‑137 is a diagnostic tumor‑suppressive miRNA <br />that targets SPHK2 to promote M1‑type tumor‑associated macrophage polarization
- Species:
- Human
- Sample Type:
- U251 cell, U373 cell
- Year:
- 2023
- Journal Name:
- Experimental and Therapeutic Medicine
Clinical observation of concentrated growth factor (CGF) combined with iliac cancellous bone and composite bone material graft on postoperative osteogenesis and inflammation in the repair of extensive mandibular defects
- Species:
- Human
- Sample Type:
- exudatum
- Year:
- 2023
- Journal Name:
- Journal of Stomatology Oral and Maxillofacial Surgery
Increased formation of neutrophil extracellular traps in patients with anti-N-methyl-d-aspartate receptor encephalitis
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Frontiers in immunology
ENO1 Binds to ApoC3 and Impairs the Proliferation of T Cells via IL-8/STAT3 Pathway in OSCC
- Species:
- Human
- Sample Type:
- CAL27 cell
- Year:
- 2022
- Journal Name:
- International Journal of Molecular Sciences
Clostridioides difficile Flagellin Activates the Intracellular NLRC4 Inflammasome
- Species:
- Human
- Sample Type:
- Caco-2 cell
- Year:
- 2022
- Journal Name:
- International Journal of Molecular Sciences
Serum IL-1β predicts de novo hepatitis B virus reactivation during direct-acting antiviral therapy for hepatitis C, not during anti-cancer/immunosuppressive therapy
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Scientific reports
Association between IL-6 and metabolic syndrome in schizophrenia patients treated with second-generation antipsychotics
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Neuropsychiatric Disease and Treatment