Human Rotavirus IgG antibody (RV-Ab-IgG) ELISA Kit

The microtiter plate provided in this kit has been pre-coated with Anti-RV-Ab-IgG. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Anti-RV-Ab-IgG and incubated. Then substrate solutions are added to each well. Only those wells that contain RV-Ab-IgG and enzyme-conjugated Anti-RV-Ab-IgG will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. 

Rotavirus is the most common cause of severe vomiting and diarrhoea among infants and young children. It is a genus of double-stranded RNA virus in the family Reoviridae. Nearly every child in the world has been infected with rotavirus at least once by the age of five. Immunity develops with each infection, so subsequent infections are less severe; adults are rarely affected. There are eight species of this virus, referred to as A, B, C, D, E, F, G and H. Rotavirus A, the most common species, causes more than 90% of rotavirus infections in humans. The virus is transmitted by the faecal-oral route. It infects and damages the cells that line the small intestine and causes gastroenteritis (which is often called stomach flu despite having no relation to influenza). Although rotavirus was discovered in 1973 by Ruth Bishop and her colleagues by electron micrograph images and accounts for up to 50% of hospitalisations for severe diarrhoea in infants and children, its importance has been underestimated within the public health community, particularly in developing countries. In addition to its impact on human health, rotavirus also infects animals, and is a pathogen of livestock.

This assay has high sensitivity and excellent specificity for detection of Human RV-Ab-IgG. No significant cross-reactivity or interference between Human RV-Ab-IgG and analogues was observed.

Matrices listed below were spiked with certain level of recombinant Human RV-Ab-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Human RV-Ab-IgG in samples.

Solid samples (feces): Take a little of the sample, put into 1 mL centrifugal tube, add 200 μL normal saline, shake properly for 2 minutes, Centrifuge and collect the supernates for assay. Samples must be free of contamination. 

Liquid samples (feces): The liquid sample is need diluted with deionized water at 1:3 (1 mL liquid sample + 2 mL deionized water), centrifuge and collect the supernates for assay. Samples must be free of contamination.