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> Human TLR-4 ELISA Kit

Human TLR-4 ELISA Kit

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Cat# ELH-TLR4-1

Size : 1PlateKit

Brand : RayBiotech

Contact local distributor :


Phone : +1 850 650 7790

Human TLR-4 ELISA KitHuman TLR-4 ELISA KitHuman TLR-4 ELISA KitHuman TLR-4 ELISA KitHuman TLR-4 ELISA KitHuman TLR-4 ELISA Kit5 Plate Kit Packaging (one box)

Human TLR-4 ELISA Kit

Human TLR-4 ELISA Kit

RayBio® Human TLR4 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
0.4 ng/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
0.4 ng/ml - 100 ng/ml
Specificity
This ELISA antibody pair detects human TLR-4. Other species not determined.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
2 fold
Accession Number
O00206
Gene Id
7099
Gene Symbols
TLR4
Protein Name / Synonyms
Toll-like receptor 4 (hToll) (CD antigen CD284)
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCancer, Inflammation, Innate Immunity
Shipping TypeBlue ice
Storage≤-20°C

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human TLR-4 ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified TLR4, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Datasheet (ELH-TLR4.pdf)
SDS (ELH-TLR4.pdf)
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Alginate oligosaccharide indirectly affects toll-like receptor signaling via the inhibition of microRNA-29b in aneurysm patients after endovascular aortic repair

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2022
    Journal Name:
    Drug Design Development and Therapy

  • Nicotine Modulates MyD88-Dependent Signaling Pathway in Macrophages during Mycobacterial Infection

    Species:
    Human
    Sample Type:
    Cell Lysate (THP-1 macrophages, nicotine and MAP infection); Tissue Lysate / Homogenate
    Year:
    2020
    Journal Name:
    Microorganisms
    PubMed ID:
    33212859

  • Permissive microbiome characterizes human subjects with a neurovascular disease cavernous angioma

    Species:
    Human
    Year:
    2020

  • Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-K?signaling

    Species:
    Human
    Year:
    2020
    Journal Name:
    Journal of Reproductive Immunology
    PubMed ID:
    32950783

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