Human Varicella zoster virus IgG (VZV-IgG) ELISA Kit
Cat# MBS2801813-5x96
Size : 5x96Tests
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Varicella Zoster Virus IgG (VZV-IgG) ELISA Kit for Human
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2801813MBS2801813 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Varicella zoster virus IgG (VZV-IgG) ELISA Kit target analytes in biological samples. The concentration gradients of… More details
Product Overview
Online Datasheet in PDFProduct Name
Varicella zoster virus IgG (VZV-IgG), ELISA Kit
Full Product Name
Human Varicella zoster virus IgG (VZV-IgG) ELISA Kit
Product Gene Name
VZV-IgG elisa kit
Species Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human VZV-IgG. No significant cross-reactivity or interference between Human VZV-IgG and analogues was observed.
Preparation and Storage
Store at 2-8 degree C
Asssay Type
Quantitive Sandwich
Sample Types
Serum, Plasma, Other biological fluids
Detection Wavelength
450nm
Sample Volume
1-200uL
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<12%
Test Principle
This assay employs a two-site sandwich ELISA to quantitate VZV-IgG in samples. An antibody specific for VZV-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVZV-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VZV-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VZV-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Assay Duration
1-4.5h
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of VZV-IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.