Human Viperin ELISA Kit
Cat# MBS454187-5x96
Size : 5x96Tests
Brand : MyBiosource
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Phone : +1 850 650 7790
Viperin (RSAD2) ELISA Kit for Human
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS454187Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Viperin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details
Product Overview
Online Datasheet in PDFProduct Name
Viperin (RSAD2), ELISA Kit
Full Product Name
Human Viperin ELISA Kit
Product Gene Name
RSAD2 elisa kit
Product Synonym Names
Cig5; RSAD2; vig1; Radical S-Adenosyl Methionine Domain Containing 2; Viperin,Endoplasmic Reticulum-Associated,Interferon-Inducible; Cytomegalovirus-induced gene 5
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Human tissue homogenates, cell lysates or other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.062ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Viperin. No significant cross-reactivity or interference between Viperin and analogues was observed.
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Viperin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Viperin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
42,170 Da
OMIM
AF026941 mRNA
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of RSAD2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for RSAD2 purchase
MBS454187 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Viperin (RSAD2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RSAD2. The ELISA analytical biochemical technique of the MBS454187 kit is based on RSAD2 antibody-RSAD2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RSAD2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RSAD2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for RSAD2. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
radical S-adenosyl methionine domain-containing protein 2
NCBI Official Synonym Full Names
radical S-adenosyl methionine domain containing 2
NCBI Official Symbol
RSAD2
NCBI Official Synonym Symbols
cig5; vig1; cig33; 2510004L01Rik
UniProt Protein Name
Radical S-adenosyl methionine domain-containing protein 2
UniProt Synonym Protein Names
Cytomegalovirus-induced gene 5 protein; Viperin; Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible
Protein Family
Radical S-adenosyl methionine domain-containing protein
UniProt Gene Name
RSAD2
NCBI Protein Information
radical S-adenosyl methionine domain-containing protein 2
UniProt Comments for RSAD2
Interferon-inducible iron-sulfur (4FE-4S) cluster-binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphosphate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T-helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins.
Show more Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with RSAD2 elisa kit
| Reference Product | PubMed Publications |
|---|---|
| ISG15 elisa kit | >23 publications with RSAD2 and ISG15 |
| MX1 elisa kit | >15 publications with RSAD2 and MX1 |
| IFIT1 elisa kit | >13 publications with RSAD2 and IFIT1 |
| USP18 elisa kit | >9 publications with RSAD2 and USP18 |
| IFIT2 elisa kit | >7 publications with RSAD2 and IFIT2 |
| DDX58 elisa kit | >7 publications with RSAD2 and DDX58 |
| IFIH1 elisa kit | >4 publications with RSAD2 and IFIH1 |
| IRF1 elisa kit | >4 publications with RSAD2 and IRF1 |
Pathways associated with RSAD2 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Cytokine Signaling In Immune System Pathway antibodies | Cytokine Signaling In Immune System Pathway Diagram |
| Immune System Pathway antibodies | Immune System Pathway Diagram |
| Influenza A Pathway antibodies | Influenza A Pathway Diagram |
| Influenza A Pathway antibodies | Influenza A Pathway Diagram |
| Interferon Signaling Pathway antibodies | Interferon Signaling Pathway Diagram |
| Interferon Alpha/beta Signaling Pathway antibodies | Interferon Alpha/beta Signaling Pathway Diagram |
Diseases associated with RSAD2 elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Liver Diseases Antibodies | >14 publications with RSAD2 and Liver Diseases |
| Inflammation Antibodies | >10 publications with RSAD2 and Inflammation |
| Nervous System Diseases Antibodies | >10 publications with RSAD2 and Nervous System Diseases |
| Influenza, Human Antibodies | >7 publications with RSAD2 and Influenza, Human |
| Necrosis Antibodies | >4 publications with RSAD2 and Necrosis |
| Carcinoma, Hepatocellular Antibodies | >3 publications with RSAD2 and Carcinoma, Hepatocellular |
| Kidney Diseases Antibodies | >3 publications with RSAD2 and Kidney Diseases |
| Cardiovascular Diseases Antibodies | >3 publications with RSAD2 and Cardiovascular Diseases |
| Myocardial Ischemia Antibodies | >3 publications with RSAD2 and Myocardial Ischemia |
| Liver Neoplasms Antibodies | >2 publications with RSAD2 and Liver Neoplasms |
Organs/Tissues associated with RSAD2 elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >24 publications with RSAD2 and Blood |
| Liver Antibodies | >14 publications with RSAD2 and Liver |
| Uterus Antibodies | >8 publications with RSAD2 and Uterus |
| Brain Antibodies | >5 publications with RSAD2 and Brain |
| Lung Antibodies | >4 publications with RSAD2 and Lung |
| Spleen Antibodies | >3 publications with RSAD2 and Spleen |
| Connective Tissue Antibodies | >2 publications with RSAD2 and Connective Tissue |
| Heart Antibodies | >2 publications with RSAD2 and Heart |
| Eye Antibodies | >2 publications with RSAD2 and Eye |
| Placenta Antibodies | >1 publications with RSAD2 and Placenta |