IgA ELISA Kit (Chicken)
Cat# OKIA00016
Size : 1x96wellplate
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
IgA ELISA Kit (Chicken) (OKIA00016)
| Datasheets/Manuals | Printable datasheet for IgA ELISA Kit (Chicken) (OKIA00016) |
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| Predicted Species Reactivity | Chicken | ||||||||||||||||
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| Product Format | In Solution | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||
| ELISA Kit Duration | 50 Minutes | ||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology IgA ELISA Kit (Chicken) (OKIA00016) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgA - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgA captured in the well. | ||||||||||||||||
| ELISA Kit Range | 12.5 ng/ml - 400 ng/ml | ||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Intended Use: The total Chicken IgA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in biological samples of Chickens. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Chicken IgA antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-CHICKEN IgA ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Chicken IgA. 7. CHICKEN IgA CALIBRATOR One vial containing Chicken IgA calibrator. | ||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Sensitivity | 2.718 ng/ml | ||||||||||||||||
| Predicted Homology Based on Immunogen Sequence | Chicken | ||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Alias Symbols | IGHA1, IGHA2, IgA, Ig alpha 1 chain C region, Ig alpha 2 chain C region |
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| Protein Name | IgA |