IgG, F(ab')2 (FITC)

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Cat# I1904-57P-2mg

Size : 2mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

I1904-57P IgG, F(ab')2 (FITC)

Clone Type

Polyclonal

Host

rabbit

Source

porcine

Isotype

IgG

Grade

Affinity Purified

Applications

FLISA IF WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:
Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Label:
Fluorescein isothiocyanate (FITC) (Molecular weight: 390D)

Absorption Wavelength:
495nm

Emission Wavelength:
528nm

Fluorochrome/Protein Ratio:
~2 moles FITC per mole of Rabbit IgG

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications

Product Type: Pab|Isotype: IgG|Host: rabbit|Source: porcine|Concentration: ~2mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). |Purity: Purified by immunoaffinity chromatography using porcine IgG coupled to agarose beads followed by solid phase adsorption(s).|Immunogen: Porcine IgG F(ab’)2 fragment|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-rabbit serum, porcine IgG, porcine IgG F(ab’)2 and porcine serum. No reaction was observed against porcine IgG F(c).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Porcine IgG F(ab’)2 fragment

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).

Purity

Purified by immunoaffinity chromatography using porcine IgG coupled to agarose beads followed by solid phase adsorption(s).

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-rabbit serum, porcine IgG, porcine IgG F(ab’)2 and porcine serum. No reaction was observed against porcine IgG F(c).

References

1. The & Feltkamp, Immunology 18: 865 (1970).