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> IgG, F(ab')2 (HRP)

IgG, F(ab')2 (HRP)

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Cat# I1904-29A-2mg

Size : 2mg

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

I1904-29A IgG, F(ab')2 (HRP)

Clone Type
Polyclonal
Host
rabbit
Source
goat
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Gt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:7000-1:50,000; Assayed against 1ug of goat IgG in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:1000-1:10,000
Immunohistochemistry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: goat|Concentration: ~2mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Goat IgG F(ab’)2 fragment|Specificity: Recognizes goat IgG, F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, goat IgG, goat IgG F(ab’)2 and goat serum. No reaction was observed against goat IgG F(c).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Goat IgG F(ab’)2 fragment
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes goat IgG, F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, goat IgG, goat IgG F(ab’)2 and goat serum. No reaction was observed against goat IgG F(c).