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> IgG, F(ab')2 (HRP)

IgG, F(ab')2 (HRP)

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Cat# I1904-57T-1mg

Size : 1mg

Brand : US Biological

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Phone : +1 850 650 7790

I1904-57T IgG, F(ab')2 (HRP)|

Clone Type
Polyclonal
Host
rabbit
Source
porcine
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Po
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:5000-1:50,000; 1ug of porcine IgG F(ab’)2 was used in a standard capture ELISA with ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:1000-1:10,000
Immunohistochemistry: 1:500-1:2500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: porcine|Concentration: ~2mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish peroxidase (HRP).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Porcine IgG F(ab')2 fragment.|Specificity: Recognizes porcine IgG F(ab’)2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, porcine IgG F(ab')2 and porcine serum. No reaction was seen against porcine IgG F(c).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Porcine IgG F(ab')2 fragment.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish peroxidase (HRP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes porcine IgG F(ab’)2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, porcine IgG F(ab')2 and porcine serum. No reaction was seen against porcine IgG F(c).