IgG (kappa chain specific) (HRP)

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Cat# I1903-37P-1mg

Size : 1mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

I1903-37P IgG (kappa chain specific) (HRP)

Clone Type

Polyclonal

Host

goat

Source

human

Isotype

F(ab’)2

Grade

Affinity Purified

Applications

E IHC WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:
Suitable for use in Western Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:50,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Applications

Product Type: Pab|Isotype: F(ab’)2|Host: goat|Source: human|Concentration: ~1mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder from 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% thimerosal. Labeled with HRP. Reconstitute with 1ml sterile ddH2O.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Human kappa chain. |Specificity: Recognizes human IgG,k. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-goat serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human kappa chain.

Form

Supplied as a lyophilized powder from 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% thimerosal. Labeled with HRP. Reconstitute with 1ml sterile ddH2O.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human IgG,k. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-goat serum. No reaction was observed against anti-pepsin or anti-goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.