rabbit Anti-IgG, Lambda (Biotin) Polyclonal Antibody

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Cat# I1904-66U-1mg

Size : 1mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

I1904-66U IgG, Lambda (Biotin)

Clone Type

Polyclonal

Host

rabbit

Source

mouse

Isotype

IgG

Grade

Affinity Purified

Applications

E IHC WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:20,000-1:100,000. Assayed against 1g of Mouse IgG in a standard capture ELISA using Streptavidin (HRP) S7973-97A and ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications

Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~1mg/ml (after reconstitution) |Form: Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Biotin.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Mouse lambda light chain|Specificity: Recognizes mouse IgG, lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse Serum. No reaction was observed against other Mouse heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Mouse lambda light chain

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Biotin.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes mouse IgG, lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse Serum. No reaction was observed against other Mouse heavy or light chain isotypes.