IgG2a (APC)

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Cat# I1904-80I-APC-100ul

Size : 100ul

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

I1904-80I-APC IgG2a (APC)

Clone Type

Monoclonal

Host

rat

Source

mouse

Isotype

IgG1

Grade

Affinity Purified

Applications

FLISA

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Applications:
Suitable for use in FLISA. Other applications not tested.

Recommended Dilution:
ELISA: 5ug/ml as coating antibody
Optimal dilutions to be determined by the researcher.

Avidity:
2.5x10e9Me-1

Recommeded Pair:
Coating: I1904-80I
Detection: I1904-83G1

Hybridoma:
Spleen cells from immunized LOU/c rat were fused with cells of the IR983F rat myeloma cell line.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: 3H2348 (LO-MG2a-9)|Host: rat|Source: mouse|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by affinity chromatography.|Immunogen: Purified IgG2a from BALB/c mice.|Specificity: Recognizes mouse gamma 2a heavy chain. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Purified IgG2a from BALB/c mice.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

Purity

Purified by affinity chromatography.

Specificity

Recognizes mouse gamma 2a heavy chain.

References

1. Lahat, G. et al. (2010) Vimentin is a novel anti-cancer therapeutic target; insights from in vitro and in vivo mice xenograft studies. PLoS One. 5(4):e10105. 2. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice. J Immunol. 185:7527-36 3. Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein. Vaccine. 24: 4102-10. 4. Hajek, A.R. et al. (2008) 12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels. J Allergy Clin Immunol. 122: 633-9.e3.