IgM (AP)
Cat# I1905-01D-1mg
Size : 1mg
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
I1905-01DIgM (AP)
Specifications
Clone TypePolyclonal
Hostgoat
Sourcemouse
IsotypeIgG
GradeAffinity Purified
ApplicationsE IHC WB
CrossreactivityHu Mo
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze
Applications:
Suitable for use in Immunomicroscopy, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
ELISA: 1:4500-1:8000
Western Blot: 1:500-1:2500
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: mouse|Concentration: ~1-2mg/ml|Form: Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA, (IgG and Protease free) 0.1% sodium azide, 50% glycerol. Labeled with alkaline phosphatease (AP).|Purity: Purified by immunoaffinity chromatography. |Immunogen: Mouse IgM mu heavy chain.|Specificity: Recognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, mouse IgM and mouse serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgM mu heavy chain.
Form
Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA, (IgG and Protease free) 0.09% sodium azide, 50% glycerol. Labeled with alkaline phosphatease (AP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, mouse IgM and mouse serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.
References
1. Avarameas and Ternyrock, immunochemistry, 32:1175, 1971.