IgM (FITC)
Cat# I1905-01Z-1.5mg
Size : 1.5mg
Brand : US Biological
Contact local distributor :
Phone : +1 850 650 7790
I1905-01Z IgM (FITC)
Clone Type
PolyclonalHost
rabbitSource
mouseIsotype
IgGGrade
Affinity PurifiedApplications
FLISA IF WBCrossreactivity
MoShipping Temp
Blue IceStorage Temp
-20°C
Applications:
Suitable for use in FLISA, Immunofluorescence and Fluorescent Western Blot.. Other applications have not been tested.
Recommended Dilutions:
FLISA: 1:10,000-1:50,000
Immunofluorescence: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Absorption Wavelength:
495nm
Emission Wavelength:
528nm
Fluorochrome/Protein Ratio:
3.6 moles FITC per mole of Rabbit IgG
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~1.5mg/ml|Form: Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC)|Purity: Purified by immunoaffinity chromatography|Immunogen: Mouse IgM mu heavy chain|Specificity: Recognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgM and mouse serum. No reaction was observed against mouse IgG or mouse IgA. Specificity was confirmed by ELISA at <1% crossreactivity against other mouse heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgM mu heavy chain
Form
Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC)
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgM and mouse serum. No reaction was observed against mouse IgG or mouse IgA. Specificity was confirmed by ELISA at <1% crossreactivity against other mouse heavy or light chain isotypes.