IL-4 Protein, Human, Recombinant (His)
Cat# NB-64-52471-10ug
Size : 10ug
Brand : Neo Biotech
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IL-4 Protein, Human, Recombinant (His)
(Synonyms: Pitrakinra, Lymphocyte Stimulatory Factor 1, Interleukin-4, IL-4, IL4, BSF-1, Binetrakin, B-Cell Stimulatory Factor 1) Copy Product InfoSynonyms: Pitrakinra, Lymphocyte Stimulatory Factor 1, Interleukin-4, IL-4, IL4, BSF-1, Binetrakin, B-Cell Stimulatory Factor 1
Catalog No. TMPJ-00035 Copy Product Info
IL-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 18 KDa and the accession number is P05112.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:SDS-PAGE: 100%; SEC-HPLC: 99%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Loaded Cynomolgus IL-4RA-Fc on Protein A Biosensor, can bind Human IL-4-His with an affinity constant of 0.33 nM as determined in BLI assay. (Regularly tested). 2. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.5 ng/ml. (QC verified); |
| Description | IL-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 18 KDa and the accession number is P05112. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-6xHis |
| Accession Number | P05112 |
| Amino Acid | His25-Ser153 |
| Construction | His25-Ser153 |
| Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.  , |
| Endotoxin | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | Pitrakinra, Lymphocyte Stimulatory Factor 1, Interleukin-4, IL-4, IL4, BSF-1, Binetrakin, B-Cell Stimulatory Factor 1 |
| Research Background | Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response. |
| Molecular Weight | 18 KDa (reducing condition) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Keywords
IL 4BSF1BSF 1
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Related Tags: IL-4 Protein, Human, Recombinant (His) chemical structure | IL-4 Protein, Human, Recombinant (His) molecular weight
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