Insulin, Recombinant, Rat

Recombinant protein corresponding to aa25-110 from rat Insulin, fused to His-Tag at N-terminal, expressed in E. coli. Swiss/Uniprot: P01323

Predicted Isoelectric Point:
5.2

Amino Acid Sequence:
FVKQHL CGSHLVEALY LVCGERGFFY TPMSGGGGSG IVDQCCTSSIC SLYQLENYCN

Predicted Molecular Mass:
13.3kD

Accurate Molecular Mass:
13kD (SDS-PAGE reducing conditions)

Subcellular Location:
Secreted

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.