Ki-67 Protein (Ki67P) Recombinant, Human

Recombinant protein corresponding to aa2815-2928 with N-Terminal His-Tag from human Ki67P, expressed in E. coli. Uniprot/Accession: P46013||Amino Acid Sequence:|MTDDKT TKIPCKSSPE LEDTATSSKR RPRTRAQKVE VKEELLAVGK LTQTSGETTH TDKEPVGEGK GTKAFKQPAK RKLDAEDVIG SRRQPRAPKE KAQPLEDLAS FQELSQTP||Predicted Isoelectric Point:|6.7||Predicted Molecular Weight:|19.3kD||Accurate Molecular Weight: |15/26kD as determined by SDS-PAGE reducing conditions||Note: The possible reasons that the actual band size differs from the predicted are as follows:|1. Splice variants: Alternative splicing may create different sized proteins from the same gene|2. Relative charge: The composition of amino acids may affect the charge of the protein.|3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.|4. Post-translation cleavage: Many proteins are synthesized as pro-proteins and then cleaved to give the active form.|5. Polymerization of the target protein: Dimerization, multimerization, etc.||Subcellular Location:|Nucleus, Chromosome||Applications:|Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||Note:|Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.