KLB siRNA (Mouse)
Cat# MBS828015-30nmol
Size : 30nmol
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
KLB siRNA – Beta-Klotho (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828015MBS828015 is an siRNA targeting KLB. Also known as KLB siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit KLB expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
KLB, siRNA
Full Product Name
KLB siRNA (Mouse)
Product Gene Name
KLB sirna
Product Synonym Names
BETAKL; Beta-klotho; BKL; BetaKlotho; Klotho beta-like protein
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
KLB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
33,142 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KLB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for KLB sirna
RNA Interference (RNAi)
Other Notes
Small volumes of KLB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for KLB. It may not necessarily be applicable to this product.
NCBI Official Full Name
beta-klotho
NCBI Official Synonym Full Names
klotho beta
NCBI Official Symbol
Klb
NCBI Official Synonym Symbols
AV071179; betaKlotho
UniProt Protein Name
Beta-klotho
UniProt Synonym Protein Names
Klotho beta-like protein
Protein Family
Beta-klotho
UniProt Gene Name
Klb
UniProt Synonym Gene Names
Betakl; BKL; BetaKlotho
UniProt Entry Name
KLOTB_MOUSE
NCBI Protein Information
beta-klotho
UniProt Comments for KLB
Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
Protein type: Membrane protein, integral
Cellular Component: membrane; plasma membrane; integral to membrane
Molecular Function: fibroblast growth factor binding; fibroblast growth factor receptor binding; hydrolase activity, hydrolyzing O-glycosyl compounds
Biological Process: fibroblast growth factor receptor signaling pathway; carbohydrate metabolic process; positive regulation of cell proliferation
Show more Protein type: Membrane protein, integral
Cellular Component: membrane; plasma membrane; integral to membrane
Molecular Function: fibroblast growth factor binding; fibroblast growth factor receptor binding; hydrolase activity, hydrolyzing O-glycosyl compounds
Biological Process: fibroblast growth factor receptor signaling pathway; carbohydrate metabolic process; positive regulation of cell proliferation
Research Articles on KLB
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with KLB sirna
| Reference Product | PubMed Publications |
|---|---|
| Fgf21 sirna | >49 publications with KLB and Fgf21 |
| Fgfr4 sirna | >28 publications with KLB and Fgfr4 |
| Fgfr1 sirna | >25 publications with KLB and Fgfr1 |
| Fgfr2 sirna | >7 publications with KLB and Fgfr2 |
| Fgf23 sirna | >6 publications with KLB and Fgf23 |
| Fgfr3 sirna | >4 publications with KLB and Fgfr3 |
| Pparg sirna | >3 publications with KLB and Pparg |
| Tnf sirna | >2 publications with KLB and Tnf |
| Fgf15 sirna | >2 publications with KLB and Fgf15 |
Pathways associated with KLB sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| ARMS-mediated Activation Pathway antibodies | ARMS-mediated Activation Pathway Diagram |
| Adaptive Immune System Pathway antibodies | Adaptive Immune System Pathway Diagram |
| Axon Guidance Pathway antibodies | Axon Guidance Pathway Diagram |
| Cytokine Signaling In Immune System Pathway antibodies | Cytokine Signaling In Immune System Pathway Diagram |
| DAP12 Interactions Pathway antibodies | DAP12 Interactions Pathway Diagram |
| DAP12 Signaling Pathway antibodies | DAP12 Signaling Pathway Diagram |
| Developmental Biology Pathway antibodies | Developmental Biology Pathway Diagram |
| Downstream Signal Transduction Pathway antibodies | Downstream Signal Transduction Pathway Diagram |
| Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies | Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram |
| Downstream Signaling Of Activated FGFR1 Pathway antibodies | Downstream Signaling Of Activated FGFR1 Pathway Diagram |
Diseases associated with KLB sirna
| Disease Name | Pubmed Publications |
|---|---|
| Liver Diseases Antibodies | >10 publications with KLB and Liver Diseases |
| Disease Models, Animal Antibodies | >9 publications with KLB and Disease Models, Animal |
| Fatty Liver Antibodies | >8 publications with KLB and Fatty Liver |
| Carcinoma, Hepatocellular Antibodies | >6 publications with KLB and Carcinoma, Hepatocellular |
| Weight Loss Antibodies | >4 publications with KLB and Weight Loss |
| Inflammation Antibodies | >4 publications with KLB and Inflammation |
| Cardiovascular Diseases Antibodies | >4 publications with KLB and Cardiovascular Diseases |
| Liver Neoplasms Antibodies | >4 publications with KLB and Liver Neoplasms |
| Kidney Diseases Antibodies | >3 publications with KLB and Kidney Diseases |
| Hyperglycemia Antibodies | >2 publications with KLB and Hyperglycemia |
Organs/Tissues associated with KLB sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Liver Antibodies | >40 publications with KLB and Liver |
| Intestine Antibodies | >11 publications with KLB and Intestine |
| Embryonic Tissue Antibodies | >3 publications with KLB and Embryonic Tissue |