KLHDC4 (Kelch Domain-containing Protein 4, DKFZp434G0522, FLJ00104) (FITC)
Cat# 128865-FITC-100ul
Size : 100ul
Brand : US Biological
128865-FITCKLHDC4 (Kelch Domain-containing Protein 4, DKFZp434G0522, FLJ00104) (FITC)
Specifications
Kelch proteins (and Kelch-like proteins) are proteins that have repeated kelch domains. Most Kelch proteins also have a BTB (defn) domain. Each kelch domain forms the blade of a propellar structure, with the repeated kelch domains forming a Beta-propellar. The structure of this Beta-propellar is known but the function is not characterised. KLHDC4 contains six Kelch repeats. There are three named isoforms.
Applications:
Suitable for use in Immunofluorescence, FLISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 1.5ug/ml
Optimal dilutions to be determined by the researcher.
AA Sequence:
MGKKGKKEKKGRGAEKTAAKMEKKVSKRSRKEETFLYNELYVYNTRKDTWTKVDIPSPPPRRCAHQAVVVPQGGGQLWVFGGEFASPNGEQFYHYKDLWVLHLATKTWEQVKSTGGPSGRSGHRMVAWKRQLILFGGFHESTRDYIYYNDVYAFNLDTFTWSKLSPSGTGPTPRSGCQMSVTPQGGIVVYGGYSKQRVKKDVDKGTRHSDMFLLKPEDGREDKWVWTRMNPSGVKPTPRSGFSVAMAPNHQTLFFGGVCDEEEEESLSGEFFNDLYFYDATRNRWFEGQLKGPKSEKKKRRRGRKEEPEGGSRPACGGAGTQGPVQLVKEVVAEDGTVVTIKQVLTAPGSAGQPRSEDEDSLEEAGSPAPGPCPRSNAMLAVKHGVLYVYGGMFEAGDRQVTLSDLHCLDLHRMEAWKALVEMDPETQEWLEETDSEEDSEEVEGAEGGVDDEDSGEESGAED
Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.

