Lyophilized from 10mM sodium citrate, pH 4.0. Reconstitute in water to a concentration of 1.0-5.0 mg/ml.
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Determined by an in vivo assay using the ob/ob mouse obesity model and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
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